کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5137222 | 1494536 | 2017 | 10 صفحه PDF | دانلود رایگان |
- Compounds from spent coffee grounds are fermented by colon human microbiota.
- Human gut fermented- non-digestible spent coffee grounds inhibited SW480 survival.
- A decrease in reduced-glutathione/oxidized-glutathione (GSH/GSSG) ratio was shown.
- Flow cytometry indicates the presence of dead cells with a DNA content lower than 2n.
- Human gut fermented- non-digestible spent coffee grounds induced apoptosis.
Gut flora-mediated non-digestible/unabsorbed fraction of spent coffee grounds (hgf-NDSCG) was evaluated for its chemopreventive effect and molecular mechanisms involved on human colon adenocarcinoma SW480 cell survival. Small intestinal digestion released bioavailable ascorbic, chlorogenic and gallic acids from hgf-NDSCG reducing oxidative stress. The hgf-NDSCG inhibited SW480 growth (LC50-19%) dose dependently by decreasing glutathione/oxidized glutathione (GSH/GSSG) ratio, indicating a cellular oxidative stress process. Flow cytometry analysis demonstrated the presence of apoptotic cells in a hypodiploid sub G0/G1-peak as a consequence of partial DNA loss. The pro-apoptotic mechanism of hgf-NDSCG was confirmed by SW480 cell toxicity induced by increased Caspase-3 activity and mitochondria dysfunction modulating oxidative stress using Annexin V and DiOC2(3) staining assays. The chemopreventive effects of spent coffee grounds (SCG) indicate that polyphenols present in hgf-NDSCG, digested and fermented by colon microbiota, can inhibit survival of human colon cancer cell and offer effective prophylactic value to prevent colon cancer.
Journal: Journal of Functional Foods - Volume 30, March 2017, Pages 237-246