کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5137266 | 1494531 | 2017 | 12 صفحه PDF | دانلود رایگان |
- In vitro B. longum growth may be supported by agave fructan.
- A single β-fructofuranosidase activity is induced in all B. longum culture fractions.
- B. longum employs the same enzyme to hydrolyze agave and inulin fructans.
- Agave fructan is hydrolyzed and internalized as oligosaccharides or fructose.
- B. longum consumes FOS first and larger fructans later in diauxic growth.
Metabolism of complex non-commercial fructans by Bifidobacteria has not been explored. In this work the production of the extracellular, cell-bound and intracellular β-fructofuranosidases by the probiotic Bifidobacterium longum subsp infantis ATCC 15697 in culture medium containing agave fructan and chicory inulin as carbon source was evaluated. The analysis of the profile of products formed in the hydrolysis of agave and inulin fructans by the three enzymatic fractions and the proteomic analysis of B. longum subsp infantis ATCC 15697 indicated the importance of B.longum_l1, an exo-β-fructofuranosidase, in the metabolism of the prebiotics studied. We found that B. longum hydrolyzes inulin and agave fructan through an exo-type mechanism involving B.longum_l1 exclusively and consumes low molecular weight fructans first in spite of their structure, suggesting that B. longum growth in these two structurally different substrates depends more on fructan molecular weight than on its chemical nature.
Journal: Journal of Functional Foods - Volume 35, August 2017, Pages 267-278