کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5137444 | 1494539 | 2016 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isolation and biochemical characterisation of angiotensin-converting enzyme inhibitory peptides derived from the enzymatic hydrolysis of cupuassu seed protein isolate
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کلمات کلیدی
ACNACETFARP-HPLCACE inhibitory activityTNBS2,4,6-trinitrobenzenesulfonic acid - 2،4،6-تری نیتروبنزن سولفونیک اسیدLC–MS/MS - LC-MS / MSangiotensin I-converting enzyme - آنزیم تبدیل آنژیوتانسین IAcetonitrile - استونیتریلTrifluoroacetic acid - اسید TrifluoroaceticLiquid chromatography-tandem mass spectrometry - طیف سنجی جرمی کروماتوگرافی مایع دو طرفهProtein hydrolysates - پروتئین هیدرولیزاتBioactive peptides - پپتیدهای Bioactivereversed-phase high performance liquid chromatography - کروماتوگرافی مایع با کارایی بالا با فاز برگشت پذیر
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The consumption of cupuassu (Theobroma grandiflorum) currently involves the pulp alone; the protein-rich seeds are viewed as leftovers by the food industry. Since some proteins may be sources of cryptic bioactive peptides (cryptides) with functions unrelated to the precursor, this study aimed to isolate and biochemically characterise angiotensin I-converting enzyme (ACE) inhibitor peptides derived from subtilisin hydrolysed cupuassu seed proteins. Eight ACE inhibitory peptides were sequenced de novo using mass spectrometry, yielding five novel sequences. The synthetic analogues were obtained and assayed for ACE IC50 and Ki determination. The peptides MVVDKLF and LDNK were competitive inhibitors, whereas FLEK and MEKHS were non-competitive. Interestingly, GSGKHVSP, which yielded the lowest IC50 and Ki values, behaved as a mixed-type inhibitor. The cupuassu cryptides presented here are not only novel alternative biotechnological tools in the field of antihypertension, but may also contribute to the reuse of the raw material as a functional food ingredient.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Functional Foods - Volume 27, December 2016, Pages 104-114
Journal: Journal of Functional Foods - Volume 27, December 2016, Pages 104-114
نویسندگان
Juliana N. da Cruz, Daniel C. Pimenta, Robson L. de Melo, João R.O. Nascimento,