Protective effect of seleno-β-lactoglobulin (Se-β-lg) against oxidative stress in D-galactose-induced aging mice

- Se-β-lg was a novel organic selenium compound chemically synthesized.
- Se-β-lg could protect oxidative stress in D-galactose-induced aging mice.
- Se-β-lg could decrease TBARS and improve SOD and GSH-Px.

The protective effect of Se-β-lg against oxidative stress was investigated in D-galactose (D-gal) induced aging mice. The mice were divided into seven groups: normal group, model group, selenium dioxide (SeO2) group, β-lactoglobulin group, low dose Se-β-lg group, middle dose Se-β-lg and high dose Se-β-lg group. The aging mice were induced by D-gal with subcutaneous injection. The ability of spatial learning was measured by Morris water maze (MWM). SOD, GPH-Px and thiobarbituric acid reactive substances (TBARS) were detected by Elisa assay and its gene expression was observed by real-time PCR. The results of MWM showed that Se-β-lg could remarkably enhance the ability of spatial learning and memory in the D-gal induced aging mice. Se-β-lg could significantly decrease the contents of TBARS and improve the protein and gene expression levels of SOD and GSH-Px. Our study revealed that Se-β-lg could enhance the activity of antioxidase to defend the D-gal induced aging.