Redox proteomics reveals the hepatoprotective effect of must from Pedro Ximénez dried grapes in aged Mus spretus mice

- Pedro Ximénez must (PXM) intake alleviated age-related hepatic damage.
- 2-DE redox proteomics identified 42 different protein spots in PXM fed mice.
- PXM polyphenols modulated protein redox signaling pathways.
- Redox regulation of transcriptionally active proteins helped liver regeneration.
- PXM offers relatively low-cost dietary changes and improves elderly health.

Aged mice consumed water supplemented with Pedro Ximénez must (PXM). After 20 weeks, histological analyses showed that PXM consumption highly alleviated the age-related hepatic damage observed in control mice, restoring the cytoplasmic appearance and normal arrangement of hepatic cords of young mice. Bidimensional electrophoresis of 5-IAF labeled hepatic proteins showed changes in the reversibly oxidized thiol status of 42 protein spots. Fourteen proteins were identified. Seven (FAM210B, RPE, SRSF1, KRT1, ATF1, ZEB1, IgM) resulted reduced and other seven (RPL29, GABPB1, ACTB, DDC, INA, PRKAR1B, ZFP738) oxidized. For 6 proteins (ATF1, RPE, ZEB1, IGM, PRKAR1B and ZFP738) the susceptibility of a particular Cys to reversible oxidation was analyze. The histological changes, the variations in the oxidation status of protein thiolic groups and the identification of specific Cys residues affected, indicated that the PXM must is acting as an antioxidant but also a pro-oxidant that might help the elderly liver to regenerate.