Achievable separation performance and analysis time in current liquid chromatographic practice for monoclonal antibody separations

In this study, the current possibilities offered by chromatography for the characterization of monoclonal antibody (mAb) are discussed. For this purpose, recently published data have been reviewed and calculations were performed to compare the maximum achievable peak capacity and related analysis times using typical samples under RPLC, IEX and SEC conditions. Carefully chosen realistic column pressure, mobile phase temperature, flow rate and column dimensions were considered for the case studies discussed through the paper.