کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5138130 | 1494600 | 2017 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The importance of system band broadening in modern size exclusion chromatography
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The importance of system band broadening in modern size exclusion chromatography The importance of system band broadening in modern size exclusion chromatography](/preview/png/5138130.png)
چکیده انگلیسی
The goal of this study was to evaluate the loss of efficiency observed with three different instruments (regular HPLC, non-optimized UHPLC and fully optimized UHPLC) offering different system variances. It appears that the new 150 Ã 4.6 mm, sub-3 μm SEC columns cannot be employed on a regular HPLC instrument, since the efficiency loss was equal to 60-85%, when analyzing mAb sample. Optimized UHPLC systems having very low extra-column volumes (typically Vec < 10 μL) have therefore to be used to properly operate these columns. Due to the instrument contribution to band broadening, the apparent efficiency of SEC columns packed with sub-2 μm particles can indeed be hampered when using inappropriate system. Considering the extra-column band broadening contribution of current UHPLC instruments, a further decrease of SEC column dimension is therefore not desired.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 135, 20 February 2017, Pages 50-60
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 135, 20 February 2017, Pages 50-60
نویسندگان
Alexandre Goyon, Davy Guillarme, Szabolcs Fekete,