کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5138322 | 1494598 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
LC-MS/MS method for the simultaneous determination of Lys-MCC-DM1, MCC-DM1 and DM1 as potential intracellular catabolites of the antibody-drug conjugate trastuzumab emtansine (T-DM1)
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Lysine-MCC-DM1, MCC-DM1 and DM1 are potential catabolites of trastuzumab emtansine (T-DM1). A convenient liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to detect these catabolites simultaneously in in vitro investigations for the first time. Protein precipitation was utilized to prepare the samples. Chromatographic separation was achieved on a Phenomenex Kinetex C18 column (100 Ã 2.1 mm, 2.6 μm) with mobile-phase gradient elution. The calibration curves of each analyte ranging from 1 to 100 nM showed good linearity (r2 > 0.995). The method was validated successfully and applied to the intracellular catabolism and regulation of T-DM1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 137, 15 April 2017, Pages 170-177
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 137, 15 April 2017, Pages 170-177
نویسندگان
Yazhong Liu, Fang Zhou, Hua Sang, Hui Ye, Qianying Chen, Lan Yao, Ping Ni, Guangji Wang, Jingwei Zhang,