کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5138350 1494601 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
RP-HPLC determination of dissociation constant using solely aqueous mobile phase
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
RP-HPLC determination of dissociation constant using solely aqueous mobile phase
چکیده انگلیسی
The proposed HPLC method using solely or nearly 100% aqueous mobile buffer as mobile phase offers fast determination of dissociation constant for compounds in relatively wide range of lipophilicity (log P from −2.26 to 2.26). The dissociation constant value for simpler chemical compounds can be determined via only 8 chromatographic runs. The number of needed chromatographic separations depends on the structural complexity of the tested compound. Moreover, the proposed method does not require a measurement of Yasuda-Shedlovsky extrapolation that includes several pKa determinations in solutions with different methanol content which speeds up considerably the procedure. The methodology is suitable for evaluation of large series of drug candidates, which can be present as complex mixtures and in small amounts.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 134, 5 February 2017, Pages 143-148
نویسندگان
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