کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5138469 1494716 2017 23 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An ultrasensitive stain for negative protein detection in SDS-PAGE via 4′,5′-Dibromofluorescein
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
An ultrasensitive stain for negative protein detection in SDS-PAGE via 4′,5′-Dibromofluorescein
چکیده انگلیسی
A highly sensitive method for brief and economical staining of proteins in 1-D and 2-D sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) by 4′,5′-Dibromofluorescein (DBF) was developed in this study. Down to 0.025-0.05 ng protein could be detected within 10 min (only 2 steps) by DBF stain, which is approximately 10-fold more sensitive than those of Eosin Y (EY) and SYPRO Ruby stains, and 20-fold more sensitive than that of imidazole-zinc (IZ) negative stain. In addition, the LC-MS/MS results indicated that the newly developed staining method is compatible with the downstream protein identification. Moreover, the mechanism of DBF stain was investigated by molecular docking.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Proteomics - Volume 165, 8 August 2017, Pages 21-25
نویسندگان
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