The synthesis of Gemini-type sulfobetaine based hybrid monolith and its application in hydrophilic interaction chromatography for small polar molecular

In this work, a novel Gemini-type sulfobetaine-based hybrid monolith was firstly fabricated by co-polymerization of a homemade Gemini-type sufobetaine, 1, 3 - bis (N - (3- sulfopropyl) - N - (methacrylic acid - 2 - hydroxy propyl ester) - N - methyl ammonium) - propane (BSMMP) with vinyltrimethoxysilane (VTMS) and tetramethoxysilane (TMOS) in present of 2′-azobis (2-methylpropionamidine) dihydrochloride (V50) as initiator. It is demonstrated that the resulting monolith possess homogeneous pore structure and good mechanical strength, and shows excellent hydrophilicity and good separation performance for a variety of polar probe compounds, including nucleoside, bases, phenol, amide. Finally, the potential application of the resulting monolith in complex sample was successfully proved by separating a BSA digest in gradient elution mode.205