Selection of an aptamer against Muscovy duck parvovirus for highly sensitive rapid visual detection by label-free aptasensor

Aptamers against Muscovy duck parvovirus (MDPV) were generated by SELEX (systematic evolution of ligands by exponential enrichment) using filtration partition and lambda exonuclease method with an affinity-based monitor and counter-screening process. The selected Apt-10 aptamer can be used to distinguish MDPV and goose parvovirus (GPV). Three-dimensional structural analysis of the Apt-10 aptamer indicated that it folded into a compact stem-loop motif that was related to its high affinity. The label-free detection method based on unmodified gold nanoparticles and Apt-10 aptamer, was developed for determination of MDPV. These results show the potential of the aptamer for rapid detection of MDPV and antiviral research.182