کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5157173 | 1500589 | 2017 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of immobilization, pH and reaction time in the modulation of α-, β- or γ-cyclodextrins production by cyclodextrin glycosyltransferase: Batch and continuous process
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
This study reports the immobilization of a β-CGTase on glutaraldehyde pre-activated silica and its use to production of cyclodextrins in batch and continuous reactions. We were able to modulate the cyclodextrin production (α-, β- and γ-CD) by immobilization and changing the reaction conditions. In batch reactions, the immobilized enzyme reached to maximum productions of 4.9 mg mLâ1 of α-CD, 3.6 mg mLâ1 of β-CD and 3.5 mg mLâ1 of γ-CD at different conditions of temperature, pH and reaction time. In continuous reactor, varying the residence time and pH it was possible to produce at pH 4.0 and 141 min of residence time preferentially γ-CD (0.75 and 3.36 mg mLâ1 of α- and γ-CD, respectively), or at pH 8.0 and 4.81 min α- and β-CDs (3.44 and 3.51 mg mLâ1).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 169, 1 August 2017, Pages 41-49
Journal: Carbohydrate Polymers - Volume 169, 1 August 2017, Pages 41-49
نویسندگان
Jéssie da Natividade Schöffer, Carla Roberta Matte, Douglas Santana Charqueiro, Eliana Weber de Menezes, Tania Maria Haas Costa, Edilson Valmir Benvenutti, Rafael C. Rodrigues, Plinho Francisco Hertz,