کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5157686 | 1500591 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Purification, characterization, and bioactivities of a polysaccharide from mycelial fermentation of Bjerkandera fumosa
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کلمات کلیدی
DPPH (PubChem CID: 2735032)MTT (PubChem CID: 64965)Galacturonic acid (PubChem CID: 439215)D-galactose (PubChem CID: 6036) - D-گالاکتوز (PubChem CID: 6036)Ascorbic acid (PubChem CID: 54670067) - اسید اسکوربیک (PubChem CID: 54670067)Bioactivities - اقدامات بیولوژیکRutin (PubChem CID: 5280805) - روتن (PubChem CID: 5280805)Purification - پاکسازیHydrogen Peroxide (PubChem CID: 784) - پراکسید هیدروژن (PubChem CID: 784)Polysaccharide - چندقندی ها یا پلیساکاریدها
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In this work, a novel polysaccharide (named DBFM3) was isolated from mycelia of Bjerkandera fumosa by DEAE-32 and Sepharose CL-6 B column chromatography. High-performance gel permeation chromatography showed that DBFM3 was homogeneous, with an average molecular weight of 1.8 Ã 105 Da. Structural characteristics of the purified fraction were investigated by high-performance liquid chromatography (HPLC), FT-IR, and NMR. HPLC analysis indicated that DBFM3 was composed of mannose, galacturonic acid, and galactose at molar ratios of 1:18.16:0.702. Spectral analysis suggested that DBFM3 had (1 â 6), (1 â 3,6), (1 â 3) linkages and pyranose conformation. Antioxidant assay in vitro showed that DBFM3 exhibited 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical scavenging activity, and has protective effect against DNA damage and damage to SH-SY5Y cells induced by H2O2. Immunological tests indicated that DBFM3 significantly increased lymphocyte proliferation in vitro. Furthermore, DBFM3 increased the proliferation of lymphocytes in the presence of concanavalin A or lipopolysaccharide as mitogen.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 167, 1 July 2017, Pages 115-122
Journal: Carbohydrate Polymers - Volume 167, 1 July 2017, Pages 115-122
نویسندگان
Di Liu, Qianwen Sun, Jing Xu, Na Li, Jianan Lin, Shan Chen, Fan Li,