Comparison of extraction and work up techniques for analysis of core and intact polar tetraether lipids from sedimentary environments

► Extraction and quantification/work up methods for IPL GDGTs compared. ► Bligh Dyer extraction performed best for extracting all IPL GDGT species. ► IPL GDGTs with a phosphate group are mainly lost during extraction and work up. ► IPL GDGTs should be quantified directly in Bligh-Dyer extracts.