Carbon and hydrogen isotope ratio characterization of methane dynamics for Fluxnet Peatland Ecosystems

Methane concentration [CH4] and stable isotope ratio values (δ13C) characterize methanogenic and methanotrophic processes within two contrasting peatland ecosystems of the Fluxnet Canada Research Network: (i) a western Canada peatland fen in northern Alberta (Fen) and (ii) an eastern Canada peatland bog in southeastern Ontario (Bog). We use carbon isotope ratio discrimination of produced methane (δ13CCH4) from the precursor carbon compounds (δ13CpreC) to estimate the relative proportions of archaebacterial acetoclastic methanogenesis (AM) and hydrogenotrophic carbonate reduction methanogenesis (HM) in these terrestrial ecosystems. The [CH4] and δ13CCH4 signatures describe contrasts in the methanogenic and methanotrophic processes between the Fen and the Bog. The differences are substantiated by stable hydrogen isotope ratio (δD) separation between the dissolved δDCH4 and co-existing δDH2O. Methanogenesis at the Fen is dominated by AM, in contrast to the Bog, which is essentially HM. We suggest that this is potentially a result of differences in type/quality of organic substrates. The trajectory of 13C enrichment in δ13CCH4 values with depth at the Bog reflects a closed system, substrate depletion effect. Our Rayleigh distillation model estimates 58-76% depletion in the source dissolved inorganic carbon (DIC).