Characterization, mutagenesis and mechanistic analysis of an ancient algal sterol C24-methyltransferase: Implications for understanding sterol evolution in the green lineage

Cloned sterol C24-methyltransferase from Chlamydmonas reinhardtii generates multiple products and possesses broad substrate specificity. The C24-methylation pathway proceeds through the Δ25(27)-olefin route which furnishes the 24β-methyl group in algal ergosterol.Download :