Elucidation of the biosynthesis of the di-C-glycosylflavone isoschaftoside, an allelopathic component from Desmodium spp. that inhibits Striga spp. development

► Di-C-glycosylflavones are biosynthesised by sequential glycosylation of 2-hydroxynaringenin. ► UDP-glucose and UDP-arabinose are the sugar donors. ► A dehydratase is required to fully dehydrate the di-C-glycosylated flavonoid intermediate. ► Specific C-glycosyltransferases are required for each glycosylation.