Purification and autolysis of the ficin isoforms from fig (Ficus carica cv. Sabz) latex

► Four ficins were purified from fig (Ficus carica cv. Sabz) latex. ► All ficins were susceptible to autolysis at high temperatures. ► The ficins autolysis rate increased with increasing storage time. ► Ficin (A) had the lowest ratio of autolytic to proteolytic activity. ► Slight ficins hydrophobic patches intensified the autolysis.