Distinct chemotypes of Tephrosia vogelii and implications for their use in pest control and soil enrichment

► Two chemotypes of Tephrosia vogelii characterised by presence or absence of rotenoids. ► Chemotype 2 lacks rotenoids but contains five new flavanones and a new dihydroflavonol. ► More than 20% field-collected specimens in Malawi were non-pesticidal chemotype 2. ► Some field-grown material incorrectly designated as Tephrosia candida instead of T. vogelii. ► Flavonoid profiling of T. candida herbarium material revealed many new glycosides.