Cloning and expression of three lipoxygenase genes from liverwort, Marchantia polymorpha L., in Escherichia coli

► Three genes homologous to plant lipoxygenase were identified from Marchantia polymorpha. ► MpLOX1 showed 11S- and 15S-LOX activities for EPA substrate and 15S-LOX activity for AA. ► MpLOX2 and MpLOX3 exhibited 15S-LOX activity for both EPA and AA.