Mass spectrometry imaging of glucosinolates in Arabidopsis flowers and siliques

► Three glucosinolates were mapped by MALDI-MSI in flower buds, sepals and siliques. ► Two sites of accumulation of glucosinolates were found: S-cells and epidermis of sepals. ► Accumulation of sulphur compounds in S-cells was confirmed by cryo-SEM-EDX.