Triterpenoid saponins from Hydrocotyle bonariensis Lam

► Five triterpene saponins were isolated from Hydrocotyle bonariensis. ► Their structures were elucidated on the basis of spectroscopic methods. ► A1- and R1-barrigenol were acylated by 2-methylbutyroyl and/or acetyl moieties. ► Two compounds showed a weak cytotoxicity against HT-29 and HCT 116 colon cancer cells.