Metabolic differentiations and classification of Verbascum species by NMR-based metabolomics

► 1H NMR metabolic fingerprinting was applied to five mullein species. ► Mulleins were divided in two groups by principal component analysis. ► Several bioactive iridoid and phenylethanoid glycosides accumulate in mulleins. ► Verbascum xanthophoeniceum and Verbascum nigrum accumulate high amounts of harpagoside.