Recent progress in liquid chromatography-based separation and label-free quantitative plant proteomics

► Label-free quantitative proteomics features are reviewed. ► Sample preparation, peptide separation, quantification and kinetic studies are discussed. ► Protein identification of non-sequenced organisms is also addressed. ► Employment of label-free LC-MS in plant science is specifically addressed. ► Perspectives in separation technology and bioinformatics/statistical analysis are presented.