Proteome analysis of wheat leaf under salt stress by two-dimensional difference gel electrophoresis (2D-DIGE)

► 2D-DIGE was used for the first time to identify salt response proteins in wheat. ► More salt concentrations reveal more detailed information salt response proteins. ► Some very important proteins were identified in the elite cultivar Zhengmai 9023. ► The results provide biochemical bases for the good salt tolerance of Zhengmai 9023.