Purification and characterization of a Bowman-Birk proteinase inhibitor from the seeds of black gram (Vigna mungo)

A proteinase inhibitor was purified from seeds of Vigna mungo (cv. TAU-1) using ion-exchange and affinity chromatography followed by gel-filtration chromatography. Biochemical characterization indicated that it belonged to Bowman-Birk inhibitor family.