Expression and characterization of the recombinant aspartic proteinase A1 from Arabidopsis thaliana

The recombinant aspartic proteinase A1 from Arabidopsis thaliana (AtAP A1) was expressed in the heterologous system Pichia pastoris in a functional soluble form. The protein consists of two chains linked by disulfide bridges and retains most of the plant specific insert (PSI) after activation.