Justicidin B 7-hydroxylase, a cytochrome P450 monooxygenase from cell cultures of Linum perenne Himmelszelt involved in the biosynthesis of diphyllin

A biosynthetic pathway for the formation of arylnaphthalene lignans like diphyllin is suggested. Justicidin B 7-hydroxylase (JusB7H) which catalyzes the last step in the biosynthesis of diphyllin was characterized as a cytochrome P450 monooxygenase from suspension cultures of Linum perenne Himmelszelt.