کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5166958 1380130 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Validation of the design of feeding experiments involving [14C]substrates used to monitor metabolic flux in higher plants
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Validation of the design of feeding experiments involving [14C]substrates used to monitor metabolic flux in higher plants
چکیده انگلیسی
The use of ratios of 14CO2 release from different combinations of positionally labelled [14C]substrates to provide information on flux through the principal pathways of carbohydrate oxidation is validated. Metabolism in Arabidopsis thaliana cell suspension cultures was unaffected by the presence of alkali used to trap respired 14CO2. Although capture of released label was incomplete, alkali traps absorbed a representative proportion of 14CO2 generated by metabolism, and reassimilation of released 14CO2 was negligible. However, caution is needed in evaluating the absolute amount of 14CO2 generated in typical feeding studies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Phytochemistry - Volume 69, Issue 17, December 2008, Pages 2920-2927
نویسندگان
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