Identification and characterization of a Bowman-Birk inhibitor active towards trypsin but not chymotrypsin in Lupinus albus seeds

The paper describes the purification, structural characterization and inhibitory properties of a trypsin inhibitor belonging to the Bowman-Birk inhibitors class from Lupinus albus L. The inhibitor inhibits two trypsin molecules simultaneously, with a Kd of 4.2 ± 0.4 nM, but not chymotrypsin. Its thermal and pH stabilities have been also assessed.