Molecular characterization of benzoxazinone-deficient mutation in diploid wheat

Accessions deficient in benzoxazinones (Bxs) were found in Triticum boeoticum, a wild A-genome diploid wheat. Molecular analyses of the five Bx biosynthetic genes (TbBx1-TbBx5) revealed that the Bx deficiency in T. boeoticum first resulted from disintegration of the TbBx1 coding sequence, followed by transcription failure and elimination of the TbBx genes.