Gene characterization, analysis of expression and in vitro synthesis of dihydroflavonol 4-reductase from [Citrus sinensis (L.) Osbeck]

A different expression pattern of the DFR gene in the flesh of both blood and blonde oranges was reported. Dfr genomic homologues as well as the promoter regions have also been isolated to find possible sequence modifications that could explain the differences noticed in dfr expression. Finally an active DFR has been expressed, this being the first report of an in vitro expression of DFR from fruit tissues.