Ecdysteroids from the ethanol extract of Diplopterygium rufopilosum

- Three new ecdysteroids were isolated from Diplopterygium rufopilosum.
- Two known ecdysteroids were also acquired from the title plant.
- The isolated ecdysteroids were evaluated in vitro for antifungal and antibacterial properties.
- They all exhibited moderate antibacterial activities against the oral pathogens.

Phytochemical investigation of the ethanol extract of Diplopterygium rufopilosum resulted in the isolation of three new ecdysteroids, (22R,24R,25S,26S)-2β,3β,14α,20R-tetrahydroxy-26α-methoxy-6-oxo-stigmast-7-ene-22,26-lactone (1), (22R,24R,25S)-2β,3β,14α,20R,26S-pentahydroxy-6-oxo-stigmast-7-ene-22,26-lactone (2), and (22R,25S)-2β,3β,14α,20R,24S-pentahydroxy-6,26-dioxo-stigmast-7-ene-22,26-lactone (3), together with two known compounds (4) and (5). Their structures were determined on the basis of spectroscopic analyses, including 1D-NMR, 2D-NMR, and HR-ESI-MS. The isolated ecdysteroids were evaluated in vitro for antimicrobial properties, and exhibited moderate antibacterial activities against the tested oral pathogens.