Chlorinated withanolides from Withania somnifera

► From Withania somnifera, one new chlorinated withanolide, 6α-chloro-5β,17α-dihydroxywithaferin A (1), was isolated together with nine known ones. ► Full NMR assignments for two chlorinated withanolides (1) and (2) were described. ► X-ray crystallography confirmed the absolute configuration of 1.