PVA immunonanofibers with controlled decay

- Chemical modification of PVA nanofibers by PEG-b enables controlled fiber decay.
- Concentration-depend scaling of biomolecules release from PVA-PEG-b fibers.
- PVA-PEG-b facilitates binding of avidin and specific functionalization by antibodies.
- Immunonanofibers with antibody enable ligand recognition and binding.

Nanofibers are utilized in crucial biomedical applications owing to their unique properties, including an enormous surface-to-volume ratio. Here, we report the biofunctionalization of polyvinyl alcohol (PVA) nanofibers by specific antibodies. This approach is built on regulated acylation of PVA nanofibers by polyethylene glycol with biotin (PEG-b) linker and sequence-specific binding of avidin-antibody conjugate. We have successfully demonstrated a chemically modified PVA-PEG-b, where the modification by the PEG-b linker resulted in the altered decay of nanofibers. Additionally, our studies on two different models indicate that the bound avidin-antibody conjugate was active. The first model was based on the binding of transferrin to immobilized anti-transferrin, and the second system was based on the recognition and binding of β1 integrin localized on mesenchymal stem cells resulting in their improved adhesion to PVA-PEG-b nanofibers. Our findings suggest that this is a versatile model for the preparation of PVA-based immuno-nanofibers whose decay can be controlled.

We report method for biofunctionalization of polyvinyl alcohol (PVA) nanofibers produced by electrospinning. The surface of PVA nanofibers was modified by polyethylene glycol-biotin (PEG-b) linker which binding of specific proteins through avidin. Additionally the system enabled scaling of PVA degradation. The system has relevance for tissue engineering and biosensor application of PVA nanofibers.240