کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5180267 | 1502545 | 2015 | 8 صفحه PDF | دانلود رایگان |
- The polymer monolith for solid-phase peptide synthesis was prepared by AGET ATRP of PEGA.
- A continuous flow technique was designed for solid-phase peptide synthesis.
- The grafting PPEGA increased the loading capacity of the monolith in the range of 0.32-0.85Â mmol/g.
- The grafted monolith gave 92% purity of the crude ACP 65-74.
- The grafted monolith gave 4-5 folds higher than Wang resins in synthesis efficiency.
The polymer monolith for solid-phase synthesis was prepared through in situ copolymerization of chloromethylstyrene and ethylene glycol dimethacrylate (PCMS-EDMA), and the obtained monolith was grafted by poly (ethylene glycol) acrylate monomer via activators generated by electron transfer atom transfer radical polymerization (AGET ATRP). The novel monolith was highly crosslinked and showed no detectable swelling in both polar and nonpolar solvents (e.g. dichloromethane, dimethylformamide, tetrahydrofuran, acetonitrile, and methanol). The grafted monolith increased the number of functional groups in the range of 0.32-0.85Â mmol/g, which resulted from side groups in each grafting polymer chain. Meanwhile, the grafted monolith showed good permeability and mechanical strength under the flow-through conditions. This monolith was derived into Wang resin and used in the synthesis of a difficult sequence-acyl carrier protein fragment 65-74 (ACP 65-74) in a new designed continuous flow equipment. The yield and purity of the crude peptide acquired from the grafted monolith reached 82% and 92%, respectively, which were higher than those by the ungrafted monolith (51% and 60%, respectively) or commercial Wang resin (51% and 61%, respectively). The synthetic efficiency on the grafted monolith in the continuous flow technique was 4-5 folds higher than on Wang resins in the manually operation conditions. Therefore, this monolithic resin showed potential effects in production scale-up.
Journal: Polymer - Volume 61, 20 March 2015, Pages 115-122