Effect of gamma radiation on dilute aqueous solutions and thin films of N-succinyl chitosan

N-Succinyl chitosan (N-SC) products with various degrees of substitution were synthesized by a direct reaction between chitosan and succinic anhydride. The susceptibility of the as-synthesized polymers to degradation upon their exposure to γ-ray radiation was investigated. The results were compared with the as-received chitosan. The size exclusion chromatographic results showed that chitosan and N-SC products in their dilute aqueous solution state were more subservient to degradation by γ-ray radiation than in their solid film state, despite the much less exposure to the radiation (i.e., 5-30 kGy for the solutions versus 20-100 kGy for the films). Increasing the radiation dose resulted in the rather monotonous decrease in the molecular weights of the polymers. Structural analyses of the irradiated polymers by Fourier-transformed infrared spectroscopy (FT-IR) and UV-visible spectrophotometry indicated the increase in the amount of carbonyl groups with the radiation dose. The formation of the carbonyl groups suggested that the radiolysis of chitosan and N-SC products occurred at the glycosidic linkages. In addition, FT-IR, elemental analysis and proton nuclear magnetic resonance spectroscopy (1H NMR) results suggested that γ-ray radiation affected both the N-acetyl and N-substituted groups on the polymer chains.