کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5209687 | 1503069 | 2015 | 6 صفحه PDF | دانلود رایگان |
Nisin grafted chitosan was prepared by using microbial transglutaminase as biocatalyst. The transglutaminase-catalyzed reaction displayed high efficiency, high selectivity, mild reaction condition and environmental friendliness. The results revealed that the degree of substitution (DS) of nisin-chitosan could be controlled by adjusting the reaction time, the reaction temperature and the molar ratio of nisin to chitosan. And nisin-chitosan in different pH showed excellent solubility. In addition, in vitro antibacterial activity assessment, nisin-chitosan with the concentration of 0.008Â mg/mL showed pronounced inhibitory effect against Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis) and Gram-negative bacteria (Escherichia coli). Furthermore, L929 mouse fibroblasts were cultured with nisin-chitosan, and the methylthiazol tetrazolium (MTT) assay showed that nisin-chitosan with the concentration from 0.005 to 0.01Â mg/mL displayed low toxicity. The results may contribute to finding the application of nisin-chitosan in pharmaceutical and food industry fields.
Journal: Reactive and Functional Polymers - Volumes 91â92, JuneâJuly 2015, Pages 71-76