کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5210310 1382885 2012 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
New copolymers graft of α,β-poly(N-2-hydroxyethyl)-d,l-aspartamide obtained from atom transfer radical polymerization as vector for gene delivery
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
New copolymers graft of α,β-poly(N-2-hydroxyethyl)-d,l-aspartamide obtained from atom transfer radical polymerization as vector for gene delivery
چکیده انگلیسی

New cationic α,β-poly(N-2-hydroxyethyl)-d,l-aspartamide (PHEA) graft copolymers were synthesized by ATRP, using diethylamino ethyl methacrylate (DEAEMA) as monomer for polymerization, yielding polycations (PHEA-pDEAEMA) able to condense DNA. Then, consecutive ATRP conditions were set up on PHEA-pDEAEMA to obtain copolymers containing also hydrophilic chains (PHEA-IB-pDMAEMA-pPEGMA) able to improve biocompatibility of polyplexes and to provide them stealth properties. Agarose gel studies showed that the copolymers effectively condensed plasmid DNA to form polyplexes. Light scattering studies were used to analyze the size and the ζ-potential of these polyplexes, showing that copolymers were able to condense the pDNA leading to the formation of nanoscale systems. The copolymers PHEA-IB-pDEAEMA showed high cytocompatibility that was improved with the presence of PEGMA units in the side chain.The transfection efficiency (luciferase) of polyplexes obtained with all copolymers was evaluated on B16F10 cell line obtaining a moderate transfection efficiency in comparison with bPEI that can be explained, supposing a low release of pDNA from polyplexes at endocellular level.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reactive and Functional Polymers - Volume 72, Issue 4, April 2012, Pages 268-278
نویسندگان
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