کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5211180 1382939 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Dye-affinity hollow fibers for β-Casein purification
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Dye-affinity hollow fibers for β-Casein purification
چکیده انگلیسی

Reactive Green HE 4BD was immobilized on polyamide (PA) hollow fibers for β-Casein adsorption from aqueous solutions. Different amounts of Reactive Green HE 4BD were incorporated on the PA hollow fibers by changing the dye attachment conditions, i.e. the initial dye concentration and the addition of sodium carbonate and sodium chloride. The maximum amount of dye attachment was obtained as 39.4 μmol/g when the hollow fibers were treated with 3 M HCl for 30 min before performing the dye attachment. β-Casein adsorption onto unmodified and dye-attached hollow fibers was investigated batchwise. The non-specific adsorption of β-Casein was low (6.0 mg/g hollow fiber). Dye attachment onto the hollow fibers significantly increased the β-Casein adsorption (80.7 mg/g). The maximum β-Casein adsorption was observed at pH 5.0. The desorptions were performed by adding 0.1 M Tris/HCl buffer containing 0.5 M NaSCN or 1.0 M NaCl to the β-Casein solutions in which adsorption equilibria had been reached. The desorption results demonstrated that the adsorption of β-Casein to the adsorbent was reversible.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reactive and Functional Polymers - Volume 68, Issue 1, January 2008, Pages 225-232
نویسندگان
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