کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5225381 1383546 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Synthesis of mono- and di-deuterated (2S,3S)-3-methylaspartic acids to facilitate measurement of intrinsic kinetic isotope effects in enzymes
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Synthesis of mono- and di-deuterated (2S,3S)-3-methylaspartic acids to facilitate measurement of intrinsic kinetic isotope effects in enzymes
چکیده انگلیسی

Kinetic isotope effects provide a powerful method to investigate the mechanisms of enzyme-catalyzed reactions, but often other slow steps in the reaction such as substrate binding or product release suppress the isotopically sensitive step. For reactions at methyl groups, this limitation may be overcomed by measuring the isotope effect by an intra-molecular competition experiment. This requires the synthesis of substrates containing regio-specifically mono- or di-deuterated methyl groups. To facilitate the mechanistic investigations of the adenosylcobalamin-dependent enzyme, glutamate mutase, we have developed a synthesis of mono- and di-deuterated (2S,3S)-3-methylaspartic acids. Key intermediates are the correspondingly labeled mesaconic acids and their dimethyl esters that potentially provide starting materials for a variety of isotopically labeled molecules.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Tetrahedron - Volume 63, Issue 22, 28 May 2007, Pages 4663-4668
نویسندگان
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