Synthesis of biotinylated sialoside to probe CD22-ligand interactions

Biotin conjugate of the most potent ligand for mouse CD22 was designed and synthesized. The key synthetic step involved the dual capability of Hanessian reaction for debenzylation and simultaneous conversion of the anomeric p-methoxyphenyl group into the corresponding phenylthioglycoside in one step. Competition enzyme-linked immunosorbent assay (ELISA) for testing the binding affinity of synthetic sialosides was developed based on this biotinylated ligand.

Biotin tagged CD22-ligand was designed and synthesized as a valuable tool for analysis of CD22 interactions and generation of B cell-binding materials.