کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5371604 1503959 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Lipopolythiourea/DNA interaction: A biophysical study
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
Lipopolythiourea/DNA interaction: A biophysical study
چکیده انگلیسی

Lipopolythioureas (LPT) are original non cationic systems representing an alternative to cationic lipids. Their high transfection efficiency prompted us to investigate further their biophysical properties, and in particular how thiourea lipids interact with DNA. The interaction of lipopolythiourea with DNA was investigated by fluorescence correlation microscopy (FCS). Influence of the lipid length and nature of the thiourea head on the thiourea/DNA interaction were studied. FCS revealed a strong interaction between lipopolythiourea and DNA, occurring at 1 equivalent of a thiourea lipid by a DNA phosphate group, and leading to a condensed plasmid state. From previous in vitro experiments, we could conclude that the lipid leading to the more condensed state of DNA was also the more efficient to transfect cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biophysical Chemistry - Volume 148, Issues 1–3, May 2010, Pages 68-73
نویسندگان
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