کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5371985 1388855 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Constitutive plasma membrane targeting and microdomain localization of Dok5 studied by single-molecule microscopy
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
Constitutive plasma membrane targeting and microdomain localization of Dok5 studied by single-molecule microscopy
چکیده انگلیسی

In the present study, single-molecule fluorescence microscopy was used to examine the characteristics of plasma membrane targeting and microdomain localization of enhanced yellow fluorescent protein (eYFP)-tagged wild-type Dok5 and its variants in living Chinese hamster ovary (CHO) cells. We found that Dok5 can target constitutively to the plasma membrane, and the PH domain is essential for this process. Furthermore, single-molecule trajectories analysis revealed that Dok5 can constitutively partition into microdomain on the plasma membrane. Finally, the potential mechanism of microdomain localization of Dok5 was discussed. This study provided insights into the characteristics of plasma membrane targeting and microdomain localization of Dok5 in living CHO cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biophysical Chemistry - Volume 136, Issue 1, July 2008, Pages 13-18
نویسندگان
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