کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5372267 | 1388871 | 2006 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Potential of cadmium sulphide nanorods as an optical microscopic probe to the folding state of cytochrome C Potential of cadmium sulphide nanorods as an optical microscopic probe to the folding state of cytochrome C](/preview/png/5372267.png)
The folding behavior of cytochrome C (Cyt-C) conjugated with CdS nanorods (CdSnr) is amenable to monitoring by bright field microscopy, the porosity and percolating behavior of such protein conjugated nanoclusters depending on the folding history prior to the conjugation. The method has been used to predict the thermal melting behavior as well as guanidine hydrochloride induced unfolding of Cyt-C. Dynamic light scattering studies indicate that the size distribution of the nanoforms widens in presence of the protein. Furthermore, there is emergence of clusters with higher conductivity and altered zeta potential. Increase of second virial coefficient of CdS nanoforms in the presence of Cyt-C (obtained from static light scattering experiments) implies presence of protein coat over the hydrophobic nanosurface. The results are supported by morphological changes observed through scanning electron microscopy (SEM). Accordingly, the X-ray diffraction pattern shows a change of crystallographic orientations of CdSnr in presence of Cyt-C.
Journal: Biophysical Chemistry - Volume 124, Issue 1, 20 October 2006, Pages 52-61