کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5372275 1503983 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Two-state irreversible thermal denaturation of Euphorbia characias latex amine oxidase
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
Two-state irreversible thermal denaturation of Euphorbia characias latex amine oxidase
چکیده انگلیسی

Thermal denaturation of Euphorbia latex amine oxidase (ELAO) has been studied by enzymatic activity, circular dichroism and differential scanning calorimetry. Thermal denaturation of ELAO is shown to be an irreversible process. Checking the validity of two-state it really describes satisfactorily the thermal denaturation of ELAO. Based on this model we obtain the activation energy, parameter T⁎ (the absolute temperature at which the rate constant of denaturation is equal to 1 min− 1), and total enthalpy of ELAO denaturation. HPLC experiments show that the thermal denatured enzyme conserves its dimeric state. The N2→kD2 model for thermal denaturation of ELAO is proposed: where N2 and D2 are the native and denatured dimer, respectively.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biophysical Chemistry - Volume 125, Issues 2–3, February 2007, Pages 254-259
نویسندگان
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