کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5398200 1505886 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A new ensemble approach based chemosensor for the reversible detection of bio-thiols and its application in live cell imaging
ترجمه فارسی عنوان
یک روش جدید گروهی مبتنی بر کیموسنسور برای تشخیص برگشت پذیر بیو تیول ها و کاربرد آن در تصویربرداری سلول زنده
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
چکیده انگلیسی
Based on an aldazine-copper chemosensing ensemble (NP-Cu2+), a new fluorescence chemosensor for the detection of biothiols (Cys, Hcy and GSH) was designed and synthesized. In aqueous solution, the ligand NP exhibited high selectivity toward Cu2+ ions by forming a 2:1 complex, accompanied with a dramatic fluorescence quenching and a notable bathochromic-shift of the absorbance band. Due to the high affinity of thiols and copper, the specific interaction of thiols (Cys, Hcy and GSH) with NP-Cu2+ ensemble led to the liberation of the NP. As the result, recovery of fluorescence and UV-vis absorbance was observed. The detection limits of NP-Cu2+ to Cys, Hcy and GSH were estimated to be 1.5 μM, 1.8 μM and 2.2 μM, respectively. The fluorescence “OFF-ON” circle can be repeated to a minimum of 5 times by the alternative addition of thiols and Cu2+, implying that NP-Cu2+ is a recyclable chemosensor for thiols. Results of fluorescence microscopy imaging suggested that NP-Cu2+ has potential to be used as a powerful tool for the detection of intracellular thiols.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Luminescence - Volume 175, July 2016, Pages 122-128
نویسندگان
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