A method for the measurement of in line pistachio aflatoxin concentration based on the laser induced fluorescence spectroscopy

Contamination of pistachio nuts with aflatoxin is one of the most significant issues related to pistachio health and expert. A fast pistachio aflatoxin concentration measurement method based on the laser induced fluorescence spectroscopy (LIFS) is proposed. The proposed method from theoretical and experimental points of view is analyzed. In our experiments XeCl Excimer laser is employed as an Ultra Violet (UV) source (λ=308 nm) and a UV-visible (UV-vis) spectrometer is used for fluorescent emission detection. Our setup is employed to measure the concentration of different type of Aflatoxins in pistachio nuts. Measurements results obtained by the LIFS method are compared with those are measured by the standard HPLC method. Aflatoxins concentrations are in good agreement with those are obtained by the HPLC method. The proposed laser induced fluorescence spectroscopy can be used as an in line aflatoxins concentrations measurement instrument for industrial applications.