کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5400183 | 1505915 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Sensitive optical detection of alkaline phosphatase activity with quantum dots
ترجمه فارسی عنوان
تشخیص نوری حساس فعالیت آلکالن فسفاتاز با نقاط کوانتومی
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
چکیده انگلیسی
A simple method has been developed to detect the activity of alkaline phosphatase (ALP) by the changing of fluorescence intensities of the quantum dots (QDs). In this system, the fluorescence intensities of the QDs were quenched by p-nitrophenol (pNP) which was produced in the process of ALP catalytic reaction. A series of linear calibration curves of the activity of ALP were obtained in different pH buffer solutions. The wide linear range was 3-1000Â UÂ Lâ1 and the detection limit was 3Â UÂ Lâ1 (S/N=3). Furthermore, the experimental conditions of biosensor were optimized, and anti-interference ability was presented. The activity of ALP was also detected in serum and the recovery of ALP in serum samples was more than 95%. The excellent performance of this biosensor indicates that it can be used in practice detection of ALP.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Luminescence - Volume 145, January 2014, Pages 330-334
Journal: Journal of Luminescence - Volume 145, January 2014, Pages 330-334
نویسندگان
Xiangling Ren, Zhenzhen Chen, Xiaoying Chen, Jing Liu, Fangqiong Tang,